DNA extraction & magnetic grain technique

DNA removal is among the important and essential experimental steps in the biomedical area. The magnetic bead DNA extraction set utilizes an unique magnetic grain and buffer system. These magnetic grains are particularly layered and have considerable affinity for the target nucleic acid under specific conditions. When problems transform, the magnetic grains will certainly launch the adsorbed nucleic acids, accomplishing the function of rapid splitting up and purification of high-quality nucleic acids. This reliable magnetic bead innovation makes it simpler and extra trusted to remove pure DNA from samples.

(LingJun DNA Extraction kit)

Magnetic grain method for DNA extraction steps:

1) Lysis: First, add the ground and broken cells to the lysis solution and allow it stand at space temperature level for 5-10 mins.

2) Binding: Then include isopropyl alcohol and magnetic bead suspension to the lysis option and mix by inverting for 5 mins. After that, put the centrifuge tube on a magnetic stand and wait up until the magnetic beads completely adsorb the target DNA prior to throwing out the supernatant.

3) Rinse: Get rid of the centrifuge tube, add rinse option and vortex to resuspend the magnetic grains. Then, put the centrifuge tube on the magnetic stand again. Dispose of the supernatant after the magnetic grains are completely taken in. Repeat this step as soon as, guaranteeing that all waste fluid is aspirated, as the residual liquid may influence subsequent experiments. Ultimately, allow completely dry for 2 minutes.

4) Elution: Get rid of the centrifuge tube, include TE buffer, vortex and pipette the magnetic grains to completely distribute them in the fluid. After that, put it at 56 ° C for 5 minutes, drinking and blending every 2 mins during this duration to make certain that the magnetic grains are evenly suspended. Ultimately, the DNA is absorbed by the magnetic grains into a new centrifuge tube through a magnetic stand.

( DNA/RNA Extraction)

DNA removal & contrast of several extraction methods

1) Discovering and understanding magnetic bead DNA removal technology: As the advantages of the magnetic bead approach in genomic DNA extraction are gradually ending up being increasingly more famous, it is recommended to pay attention to the growth of this innovation and learn how to run and optimize the magnetic grain DNA extraction speculative process.

2) Understand high-throughput and automatic equipment: As the organic industry’s need for efficiency and automation boosts, experience with and proficiency of appropriate high-throughput DNA removal tools and automated systems can improve experimental effectiveness and accuracy.

3) Safe operation and experimental performance: Thinking about the danger of direct exposure to harmful reagents using traditional methods, it is advised to use the magnetic grain method for DNA extraction to reduce the injury to human health throughout speculative procedures and enhance speculative effectiveness and security.

4) Expansion and application of study fields: In addition to genomic DNA removal, magnetic bead methods are likewise widely used in illness discovery, hereditary diagnosis, forensic identification and various other fields. It is recommended to discover additionally the potential applications of the magnetic grain technique in different organic fields and add more opportunities to the development of the organic market.

( Lingjun Products)

Vendor Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction kit.
We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need dna extraction, please feel free to contact us at sales01@lingjunbio.com.

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